News
Date: July 2023
Subject: Discontinuation of BiSulfite Library Preparation Kits from July 2023
Starting from July 2023, we will discontinue our BiSulfite library preparation kits. However, we have an exciting alternative to offer – the EnzymaticMethylome library kits. These kits not only provide TruSeq-compatible library preparation but also include bisulfite conversion.
Key Points:
- BiSulfite library preparation kits will be discontinued from July 2023.
- Consider using our EnzymaticMethylome library kits as an alternative.
- These kits are TruSeq compatible and include bisulfite conversion.
- Available for online ordering through our system.
If you want to incorporate the EnzymaticMethylome library kit into your projects, they are now available via our online system. Please contact us for further information or experimental planning.
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Date: April 2023
Subject: New status of the MP-GC since January 2023
Since January 2023 the Max Planck Genome centre Cologne (MP-GC) receives limited financial support from the MPG headquarters to be able to serve MPG scientists as a core facility for long-read and short-read next generation sequencing (genomics, transcriptomics) and cell sorting, especially for Max Planck Institutes which provide a significant additional financial contribution to keep MP-GC alive (MPI Plant Breeding, Cologne, MPI Molecular Microbiology, Bremen and MPI Chemical Ecology, Jena).
In case you are interested to work with us, we will decide about it from case to case (contact to MP-GC!)
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Date: January 2021
Subject: No central MPG funding beyond 2020
From 2010 until 2020 the Max Planck Genome centre Cologne (MP-GC) received financial support from the MPG headquarters to be able to serve MPG scientists as a core facility for next generation sequencing. As finally decided in December 2020, no central support will be provided beyond 2020.
The MP-GC currently has to adjust to the new financial situation and will effectively stop to provide any support on new projects in 2021. All active projects will of course be finished, although it may take a bit longer due to reduced personnel as an immediate effect. In case you additionally want to stop processing an active project, please contact us!
In the future we will provide our services to a limited set of institutes and/or departments who agreed to make financial contributions to keep the MP-GC services vital in a next phase.
The MP-GC team wants to thank for your interest and trust in our service in the recent years!
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Date: May 1st 2020
Subject: Price reductions for sample treatments and library preparation
Dear colleagues,
The MP-GC has updated the price list for next generation sequencing, new prices are valid for all projects created in MPGC-SOS (Max Planck Genome centre Cologne -Sequencing Order System) since 01. May 2020. Due to optimization of working procedures and further lab automation we were able to reduce the costs for many sample treatments and library preparation protocols. In detail, the prices for the following items have been reduced:
• gDNA isolation (bacterial cells)
• gDNA isolation (plants)
• gDNA isolation (high molecular weight)
• total RNA isolation
• genomic DNA clean-up
• DNAse treatment incl. clean-up
• RNAse treatment incl. clean-up
• Covaris fragmentation incl. clean-up
• RNA library incl. polyA enrichment
• 95-plex RNA library incl. polyA enrichment
• RNA library incl. rRNA depletion
• Ultra Low Input RNA library
• small RNA library
• DNA library
• 95-plex DNA library
• Ultra Low Input DNA library
• ChIPSeq library
All other prices (e.g. sequencing and bioinformatics) remain constant. To access our complete price list, please follow this link (user login for MPGC-SOS required).
For comparison with other sequencing offers, please keep in mind that our prices are “final” prices for all Max-Planck members, no VAT (MwSt) will be added.
Please contact us in case of questions!
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Date: April 20th 2020
Subject: End of reduced working mode due to COVID-19 pandemic
After operating in reduced mode due to COVID-19 in the past weeks, Genome Centre returns to its standard working mode now. Please feel free to place new sequencing requests without prior consultation!
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Date: March 23rd 2020
Subject: Reduced mode due to COVID-19 pandemic
NEW: Due to the new COVID-19 pandemic we operate in reduced mode until April 16th, 2020 (currently announced end of CORONA measures in Northrhine-Westphalia). Please get in touch with us, before sending new samples to Genome Centre. During this period we operate with reduced activity and a reduced number of staff. We will provide an update in case there are additional changes in the current work mode.
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Date: January 2020
Subject: PacBio sequencing with Sequel and Sequel II
The MP-GC now offers PacBio sequencing on two different devices: Sequel and Sequel II. Both devices work with the same sequencing technology and same libraries, but data output for Sequel II SMRT cells (25-100 Gb) is significantly higher than for Sequel SMRT cells (4-10 Gb). Although it may seem uneconomic at first sight, we will continue sequencing on PacBio Sequel for projects with limited input material, as sequencing on this device works at significantly lower concentration. For further questions regarding PacBio sequencing, feel free to contact us!
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Date: 25. November 2019
Subject: Limited sample receiving capacities during Christmas time
We wish a merry Christmas time to all our customers and collaborators!
Please do not send any samples or libraries from 18.12.2019 until 06.01.2020, as we cannot guarantee proper sample handling within this period.
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Date: June 2019
Subject: High throughput RNA and DNA isolation service
MP-GC now offers a service for RNA and DNA isolation from various inputs in a 96-well scale to cover the whole pipeline from tissue input to NGS library preparation followed by sequencing. We recommend to get in touch with MP-GC before submitting samples, since we will provide customers with specially equipped 96-deep well plates so that MP-GC can start directly without additional interference after submission of samples.Our prices for this service can be found here.
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Date: November 2018
Subject: PacBio Sequel: New chemistry – new prices
PacBio has released its third update for the Sequel! With the release of the sequencing chemistry 3.0, the output per SMRTCell has increased significantly. The last runs show up to 10 Gb/SMRTCell output for genomic DNA sequencing and up to 20 Gb/SMRTCell for Isoseq and amplicon sequencing! This is a duplication in output. Unfortunately, the price for the new chemistry has also increased and while we tried over several releases to keep the price on the same level, this is not possible anymore. Our new prices can be found here.
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Date: September 2018
Subject: RiboZero rRNA depletion kits will be discontinued by the end of the year
Illumina will discontinue the RiboZero kits for rRNA depletion by the end of the year. We will order some to keep in stock and will look for a replacement in the mean time. In case you are planning a large rRNA depletion project until the end of the year or want to create data that is comparable to previous rRNA depletion projects, please contact us us for further information/experimental planning.
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Date: July 2018
Subject: TPase-based DNA library preparation
We now offer a transposase (TPase)-based DNA library preparation assay. This fast and high-throughput DNA library preparation method is especially suited for large screening projects, due to low input amounts (min. 20 ng/sample) and costs (15 €/sample for up to 95 samples and 9 €/sample for > 95 samples). Please note: Our cost calculations do not cover individual sample handling (e.g. clean-up or volume reduction) or repetition in case of sporadic outages! Please feel free to contact us us for further information/experimental planning.
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Date: June 2018
Subject: Nanopore sequencing
After an initial training phase we now offer and routinely perform Nanopore sequencing on the GridION X5 (Oxford Nanopore). If you are interested in using this sequencing technology, please contact us us for further information/experimental planning.
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Date: 1. May 2018
Subject: New protocol for Illumina DNA libraries
Starting 1. May 2018, we will be using a new protocol for the preparation of standard Illumina DNA libraries. The main difference of the new protocol is the method of DNA fragmentation: we will perform random enzymatic fragmentation instead of sonication now. If you have further question or if you are planning an experiment to be directly comparable to data from an older project, please contact us us in advance.
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Date: 1. February 2018
Subject: Limited sample receiving capacities during carnival time
Please remember: From 8. – 13. February 2018 there is carnival season in Cologne!
We HIGHLY discourage from sending any samples within this period!
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Date: 1. January 2018
Subject: Personnel changes at the Max Planck Genome Centre Cologne
With the retirement of Richard Reinhardt by the end of 2017, Bruno Hüttel has become the new head of the Max Planck Genome Centre Cologne. Bruno Hüttel used to be responsible for the wet lab management, his former position has now been staffed with a new team member: Ronny Brandt. The MP-GC team thanks Richard Reinhardt for his long-time commitment!
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Date: 11. December 2017
Subject: Limited sample receiving capacities during Christmas time
Dear NGS user,
We wish a merry Christmas time to all our customers and collaborators!
Please note: From 20.12.2017 until 02.01.2018 no new samples or libraries can be handed in, as we cannot gurantee proper sample handling within this period.
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Date: 16. October 2017
Subject: New Ordering System
Dear NGS user,
Today, we have been launching our new ordering system: Max Planck Genome Centre Cologne – Sequencing Order System, short MPGC-SOS. You will find it at https://mpgc-sos.mpipz.mpg.de . The old NGSOrderDB will be discontinued and is from today on only available to finish on-going projects. All new projects from today on will be managed viea the new system. If you have questions, do not hesitate to contact us.
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Date: 11. October 2017
Subject: Public Holidays in NRW in October/November
Dear NGS user,
This year is “Luther year”, so even catholic Northrhine Westphalia will celebrate Tuesday, 31st October as a public holiday in addition to “Allerheiligen” on the 1st November. As a consequence, the Genome Centre is closed both days and can not receive samples. On Monday that week (30.10.2017), the sample receiving capacity is extremely low. So please do not send samples to arrive before Thursday of that week.
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Date: 27. September 2017
Subject: PacBio control DNA in PacBio data
Dear NGS user,
To ensure the data quality on the PacBio Sequel, Pacific Biosciences offeres a control library to be sequenced with the normal sample. We have started to include this control library to be able to ensure best performance. However, some reads of your dataset may originate from this spike in. Please find the fasta file for download here .
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Date: 1. September 2017
Subject: Lisa is back, MiSeq, additional sequencing conditions
Dear NGS user,
A few things changed on the 1st of September:
Lisa Czaja-Hasse is back from maternity leave
Lisa Czaja-Hasse was gone for a year and is now back at her position, so you can contact her instead of Janina Fuss for all types of inquries. Or you could use our new, general e-mail address mpgc@mpipz.mpg.de for inquiries.
The Illumina MiSeq retired on 1st September
Due to very unsatisfying results on most MiSeq runs due very long standing Illumina chemistry problems and a consequently low user request for MiSeq runs with 2×300 bp, we decided to shut down the MiSeq instrument. We will finish the ongoing projects but no new projects will be accepted from 1st September. We are very sorry to do so. In case you want to sequence amplicons, please contact us to discuss alternatives on the HiSeq2500 with 2×250 bp.
New run mode(s)
We now have the possibility to offer addtional run modes: 2×75 bp on the HiSeq3000 and 2×150 bp/2×100 bp on the HiSeq2500 Rapid mode. You can request all run modes as Gb-amounts. However, the waiting times might be longer (up to eight weeks for sequencing) for the HiSeq2500, as we will have to fill the runs. The Hiseq2500 is recommended by 10x Genomics for sequenicng de novo projects and it has a non-patterend flow cell compared to the HiSeq3000. You can find the prices for those run modes here .
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Date: 17. July 2017
Subject: New prices, input amounts, instruments
Dear NGS user,
We now offer a range of new services that were tested here since Spring of 2017.
New RNA library prep protocol established – new input amounts for all kits
We have introduced a new library preparation protocol for RNA library preparations, which allows us to reduce the minimal input for RNA libraries with polyA enrichment and with rRNA depletion down to a 10 ng. We also provide now a minimum (lowest limit of each kit) and a recommended input amount, which ensures highest sequence diversity. If anyhow possible, follow our recommendations as:
A) For sequencing, more input will always result in a more complete (diverse) picture of the genome and a more reliable snap shot of the transcriptome at sampling time.
B) For (quantitative) comparative sequencing approaches, the same input amount must has to be used for all samples to ensure comparability! So please indicate “comparative gene expression analysis” for comparative RNA approaches as aim of project and keep in mind, that the input we use is then adjusted to the sample with the lowest input amount. In case you use DNA as template, indicate the need for comparative quantification in the comment field.
C) For any comparative approach, it is best practice to go for “PCR-free” library construction, if enough sample (min: 500 ng, recommended: 1500 ng) is available.
New instrumentation for long range sequence information available
PacBio SEQUEL
We have established sequencing with the PacBio Sequel system to replace the RSII, which increases the output per SMRT Cell significantly at comparable read lengths. All new projects are sequenced on the Sequel. The output per SMRT Cell has increased to aon average of 3 Gb (~2-6 Gb). However, we can only guarantee this output when one library is sequenced several times. PacBio recommends titrating of the optimal loading concentration for each library and estimates 3 SMRTCells to determine optimal loading concentrations. When only one SMRTCell is ordered, it will most likely not be loaded optimally and therefore the result might be below average.
In case you need less data or already have data created on the RSII, please contact Bruno Hüttel us for further advice. The RSII and the Sequel use different post run processing pipelines (SMRTLink and SMRT Analysis Suite), so data generated from both has to be analyzed separately until obtaining error corrected fastq/fasta files.
We will no longer offer the large AND extra large libraries for DNA, only libraries > 10 kb. The Sequel loading is not as size sensitive as the one for the RSII, so we can load broader size ranges and do not need to size the libraries anymore.
The full transcriptome can be sequenced on one SMRTCell due to the reduced size sensitivity. Therefore, we changed our IsoSeq libraries as well to one price for a full IsoSeq library with the option to size for larger fragments in addition.
10X GENOMICS linked read sequencing
The 10x genomics chromium controller allows for linked read sequencing of genomic DNA. This means, each fragment of high molecular weight DNA is used as a template in an isothermal amplification within an emulsion reaction, also including one of 4.2 mio individual barcodes. With the help of these barcodes, each read from an standard Illumina library containing these barcodes (GEMs) can be traced back to the strand of DNA it came from. This means, depending on your fragment size, you can gain location information over 50 -100 kb with short read sequencing. This is very useful for haplotype phasing, re-arrangement detection, de novo genome sequencing and genome polishing. It is especially suitable for genomes > 200 Mb, optimal for genomes > 1 Gb. If you want to know how this is done, please refer to this very nice, introductory video and if you want to know what else can be done, please visit the 10x Genomics homepage. At the moment, the set-up and software are designed for human and human-like genomes concerning ploidy, heterogeneity and phasing. It is possible to work with other genomes as well, but might require some further optimization.
To request for 10x linked reads, please chose 10 x linked read library in the NGS Order DB. Select 2 × 150 bp as run conditions, as this is the only supported read length for 10x libraries. We also offer a special bioinformatics package for linked read libraries to help with assembly, phasing and variant calling. For further questions regarding read length, feasibility and input requirements, please contact Janina Fuß.
femto Pulse for ultra low input and high molecular weight quality control
The femto Pulse is the successor og the fragment analyser, measuring quantities in the fg range and fragment sizes larger than 150 kb due to its pulse field function. Together with the PacBio Sequel and the 10 x Genomics, the femto Pulse completes the expanded field of high molecular weight DNA sequencing.
New services and quantities available due to frequent requests
We often have requests for additional treatment of samples, including measures to increase quality of the input, but also the extraction of optimal input DNA for different types of sequencing including high molecular weight DNA. We have added a range of the most common requests to our standard procedures. You’ll find details here.
We have added the possibility to request for full lanes on our HiSeq 3000 now, so please indicate this in the comment field. For non-standard read lengths, there is always the option to request a full run. We have standard prices now for 50 bp – single read sequencing on the HiSeq2500 and 3000, prices for all other read lengths will be made available on request.
These are on average slightly cheaper than requesting Gb or reads, but we do not guarantee output on lanes or flow cells! So we will pool the libraries for this lane equimolarly and in case the libraries differ in performance, we will not automatically re-sequence those. Illumina quality criteria apply for the definition of a failed lane/flow cell.
We have stopped offering MiSeq runs by the Gb mode, we only offer to sequence libraries in full flow cell mode at the Illumina list price. We will follow your request of loading concentration and PhiX spiking. If you do not provide specific instructions, we will follow Illumina’s recommendation and start with 12-14 pM loading concentration and 40 % PhiX spike-in. Please be aware that Illumina doesn’t guarantee quality specifications on the MiSeq runs with (16s/18s) amplicons, so the data output will be dispatched as it is, independent of quality and quantity.
All details on prices you will find here, while you can find information on the new instrumentation here , and the new loading concentrations in addition here.
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Date: 15. December 2016
Subject: Limited sample receiving capacities during Christmas time
Dear NGS user:
We wish a merry Christmas time to all our customers and collaborators!
Please note: From 23.12.2016 until 02.01.2017 NO new samples or libraries can be handed in. The absolute last date for sending in samples will be the 21.12.16 for everyone not handing in the samples personally!
At any timepoint later than that, we can’t gurantee the proper handling of the samples.
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Date: 01. September 2016
Subject: Updated Price List for NGS
Dear colleagues,
The MP-GC has updated the price list for next generation sequencing, new prices are valid for all projects created in NGS OrderDB since 01. September 2016.
Please find below a short summary of important changes in the next paragraph. For more details, please check the new price list here.
Due to optimizations in library preparation protocols, we were also able to reduce the minimum input material for some preparations significantly here.
Major changes for Illumina sequencing technology are:
• We have reduced our pricing per Gigabase for the HiSeq systems by 30 %.
• We were able to optimize the PCRfree library preparation protocol for DNA libraries, so we can offer PCRfree libraries at the same price as standard DNA libraries. The required DNA amount is still higher (2,5 µg DNA / sample).
Major changes for PacBio sequencing technology are:
• We observed a significant increase in read length and read numbers when performing damage repair for all PacBio libraries above 10 kb size, so we adopted this as a standard.
For comparison with other sequencing offers, please keep in mind that our prices are “final” prices for all Max-Planck members, no VAT (MwSt) will be added.
Please contact us in case of questions!
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Date: 23. June 2016
Subject: Example for full length transcriptome PacBio sequencing
cDNA Library Enrichment of Full Length Transcripts for SMRT Long Read Sequencing
The utility of genome assemblies does not only rely on the quality of the assembled genome sequence, but also on the quality of the gene annotations. The Pacific Biosciences Iso-Seq technology is a powerful support for accurate eukaryotic gene model annotation as it allows for direct readout of full-length cDNA sequences without the need for noisy short read-based transcript assembly. We propose the implementation of the TeloPrime Full Length cDNA Amplification kit to the Pacific Biosciences Iso-Seq technology in order to enrich for genuine full-length transcripts in the cDNA libraries. We provide evidence that TeloPrime outperforms the commonly used SMARTer PCR cDNA Synthesis Kit in identifying transcription start and end sites in Arabidopsis thaliana. Furthermore, we show that TeloPrime-based Pacific Biosciences Iso-Seq can be successfully applied to the polyploid genome of bread wheat (Triticum aestivum) not only to efficiently annotate gene models, but also to identify novel transcription sites, gene homeologs, splicing isoforms and previously unidentified gene loci.
Link publication
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Date: 14. December 2015
Subject: Limited sample receiving capacities during Christmas time
Dear NGS user:
We wish a merry Christmas time to all our customers and collaborators!
Please note: From 23.12.2015 until 04.01.2016 NO new samples or libraries can be handed in. Please do not send any samples within this period!
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Date: 30. November 2015
Subject: MP-GC services 2016/2017
Dear NGS user:
Within the last weeks several colleagues from different MPI`s contacted us with concerns that the MP-GC has to close its service soon. This information is really wrong! The MP-GC has been evaluated and it’s budget been proven until end of December 2017. So there is no question that we provide the service according to this application. All MPI scientists are highly welcome to use our services and for sure there is no need to change to any commercial service provider now.
We have presently implemented the new HiSeq 3000 sequencing platform, providing a much higher capacity, been much faster in running time, offering even increased quality compared to older Illumina systems and last not least pushing the standard read length up to 150 bp.
If you have any further question, please feel free to contact us!
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Date: 15. November 2015
Subject: Updated Price List for NGS
Dear colleagues,
The MP-GC has updated the price list for next generation sequencing, new prices are valid for all projects created in NGS OrderDB since 15. November 2015.
Some price correction were necessary, to adopt to reality, as we had a drastic increase in spring 2015 due to changed US-Dollar/Euro conversion. Nevertheless, most of the Illumina prices have been even reduced, because of the installation of the new powerful HiSeq 3000 system. Following, you will find a short summary of important changes, for more details, please check the new price list here.
For comparison with other sequencing offers, please keep in mind that our prices are “final” prices for all Max-Planck members, no VAT (MwSt) will be added.
Major changes for Illumina sequencing technology are:
• We now offer sequencing on the HiSeq 3000 platform! This new instrument generates longer reads (150 bp) in shorter time.
• We introduce routinely 2 × 250 bp paired end sequencing in so called “rapid mode” on HiSeq 2500.
• Our pricing scheme for Illumina sequencing has been modified from a read based system (counting number of reads) to a volume based system (counting number of Gbases). This will allow easier comparison with other projects, simplify ordering of a specific data coverage in Gb and last not least, stands for higher transparency. Consequently, we have calculated one unique price per Gbase for Illumina HiSeq sequencing at fixed 70 €/Gb.
• 150 bp read length will become the new standard and is also fixed to 70 € per 1 Gbase for single and paired end reads. Ordering 100 bp reads is limited to the next three month to finalize or fill-up on-going projects. For the future, we need to get high order-volumes of identical read length to fill up the HiSeq 3000 flow-cell a.s.a.p., therefore, we need again mainly one unique read length.
• Re-sequencing for matching the requested data volume, is the limiting situation for short project run times. To reduce project run times, we have opened the tolerance window for projects to be judged as “done” from 15 % to 30 %. Regarding costs, we stick to the same rules as before: only the generate data amount (within the tolerance window) will be charged. If more data is generated, only the ordered volume will be charged, nothing more. So it’s still easy to sum up the max. costs for your project, when ordering. Consequently, you might increase your order volume to get enough data done within the first run. Regarding the cost reduction, we think this is a time-saving and economically reasonable approach for all parties.
• Following frequent requests, mate pair library preparation has been added to our list of service offers.
Major changes for PacBio sequencing technology are:
• Due to increased demand, we now offer genomic DNA clean-up service.
• Full length transcriptome sequencing is now supported in a full service package. For details, please refer to the price list.
Please contact us in case of questions!
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Date: 18. June 2015
Subject: 2 × 250 bp sequencing on HiSeq2500 platform
Dear NGS user:
Upon request, we now also offer 2 × 250 bp sequencing on our HiSeq2500 platform. Please note: sequencing in this read length can only be performed in a rapid run mode with reduced capacities of 2 lanes per run. Currently, the minimal order quantity is 1 lane (approx. 140 million reads). For further information, please contact us!
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Date: 1. June 2015
Subject: 150 bp sequencing not offered any more
Dear NGS user:
We no longer offer 150 bp sequencing on the HiSeq2500 platform. Alternatively, 250 bp sequencing will be available soon.
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Date: 15. May 2015
Subject: PCR-free DNA libraries
Dear NGS user:
Due to many requests within the last weeks, please find here further information regarding PCR-free library preparation.
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Date: 5. February 2015
Subject: Limited sample receiving capacities during carnival time
Dear NGS user:
Please remember: From 12. – 18. February 2015 there is carnival season in Cologne!
We HIGHLY discourage from sending any samples within this period!
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Date: 9. January 2015
Subject: 454 sequencing no longer offered on regular basis
Dear NGS user:
Due to the rapidly decreasing number of requests throughout 2014, in 2015 454 sequencing will no longer be offered on a regular basis. Nevertheless: If you have a specific project request requiring 454 sequencing, please contact us to discuss what we can do for you!
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Date: 26. November 2014
Subject: Limited sample receiving capacities during Christmas time
Dear NGS user:
First of all: We wish a merry Christmas time to all our customers and collaborators!
Please note: From 20.12.2014 until 04.01.2015 NO new samples or libraries can be handed in. Please do not send any samples within this period!
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Date: 22. September 2014
Subject: Update of NGS OrderDB requires emptying browser cache
Dear NGS user:
We recently updated NGS OrderDB to make it more convenient for you. This update makes it necessary to empty the cache of your browser before starting the updated appliance. Please click here for guidance on how to do so.
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Date: 2. September 2014
Subject: Updated price list for NGS sequencing
The MP-GC has updated prices for NGS sequencing!
The new price list is valid for all projects created after 2nd September 2014 and can be found here.
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Date: 11. August 2014
Subject: Welcome our new colleague
The MP-GC welcomes a new colleague:
Last week our new bioinformatician Palani Kannan Kandavel started working at MP-GC. After an initial training phase, he will support the MP-GC with data analysis.
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Date: 21. July 2014
Subject: Welcome our new colleague
The MP-GC welcomes a new colleague:
Last week our new secretary Gabriele Breun started working at MP-GC. For general requests she will be available on daily basis between 10 am and 14 pm.
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Date: 11. July 2014
Subject: Further delay of 250 bp paired end sequencing
Dear NGS user:
Unfortunately, the recent problems with the MiSeq platform (see last entry) have not been solved so far. After the 3rd (!) repair, the device still is not free from defects! We hope this problem can be solved within the next days and will continue with routine 250 bp paired end sequencing as soon as possible.
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Date: 24. June 2014
Subject: Delay of 250 bp paired end sequencing
Dear NGS user:
Due to recent technical problems with the MiSeq sequencing platform all current 250 bp paired end sequencing requests will be delayed. The device has been in repair last week and currently is in repair again. We hope this problem can be solved rapidly and will continue with routine 250 bp paired end sequencing as soon as possible.
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Date: 25. April 2014
Subject: Alteration of sequencing read length
Dear NGS user:
Because of recent technical sequencing problems on the HiSeq2500 platform MP-GC has to reduce the standard sequencing read length from 100 bp to 98 bp for the next future.
For your understanding: MP-GC routinely uses 8 bp instead of 6 bp indices (as intended by Illumina) to increase multiplexing of libraries. This on the other hand reduces the possible read length by 2 bp. In the past we found a way to avoid this shortage by 2 bp, which is not supported by Illumina and which currently does not work anymore.
We are currently developing a strategy to get back to a 100 bp standard read length, but this will take some time. If the shortage by 2 bp causes problems to any of your ongoing projects please contact us to discuss how we can help to bioinformatically solve this problem.
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Date: 17. April 2014
Subject: Happy Easter!
The MP-GC team wishes happy Easter holidays to all customers and collaborators!
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Date: 3. April 2014
Subject: Automated upload of large projects into NGS OrderDB
Dear NGS user:
To save time when creating large (> 25 libraries) sequencing projects you now have the opportunity to fill out a corresponding excel sheet and send it to us for an automated upload into NGS OrderDB. For details click
here.
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Date: 11. March 2014
Subject: Illumina 150 bp reads no longer offered as standard service!
Dear NGS user:
Our standard sequencing read length for the HiSeq2500 platform are 100 bp and since March 2013 also 150 bp in rapid mode. After one year experience, we have to notice, that 150 bp sequencing at MP-GC has been used only quite rarely. As the rapid mode is associated with high additional time-consuming efforts, we will consequently no longer offer 150 bp sequencing in rapid mode on the HiSeq 2500 system. Nevertheless: If you have a specific project request requiring this read length, please feel free to contact us for help.