Instrumentation

Instruments for sequencing

Revio (Pacific Biosciences)	NEW @ MP-GC Cologne: higher output, higher throughput single-molecule sequencing long-read high-fidelity (>=Q20) sequencing (15-20 kb) many applications (e.g. de novo genome sequencing, genome finishing, base modification analysis, CG methylation, full-length cDNA sequencing, transcript isoform sequencing, …) 15-fold higher throughput per week 3-fold higher output per sequencing unit (SMRT cell) 12-fold arrayed insert sequencing (16 S rRNA, 10x Genomics barcoded full-length cDNAs, poly-A RNAs)
Sequel IIe (Pacific Biosciences)	single-molecule sequencing long-read high-fidelity (>=Q20) sequencing (15-20 kb) many applications (e.g. de novo genome sequencing, genome finishing, base modification analysis, full length cDNA sequencing, transcript isoform sequencing, …)
NextSeq 2000 (Illumina)	short-read sequencing (2 × 50 to 2 × 300 bp) up to 330 Gb per run paired end sequencing many applications (e.g. de novo genome sequencing, genome re-sequencing, transcriptome sequencing (RNA-Seq), ChIP sequencing, small RNA sequencing, enzymatic methylome, …)
GridION X5 (Oxford Nanopore Technologies)	single-molecule sequencing sequencing of very long reads data output: 3-10 Gb/Nanopore cell sequencing of up to 5 Nanopore cells in parallel many applications (e.g. de novo genome sequencing, genome finishing, metagenome sequencing, full length cDNA sequencing, …)

Instruments for single cell sequencing

BD FACSAria Fusion Cell Sorter (BD Biosciences)	high speed cell sorter and analyzer multicolor detection (355 nm, 405 nm, 488 nm, 561 nm, 640 nm) sorting into tubes and multiwell plates
Chromium Controller (10x Genomics)	a droplet-based system to: a) label large DNA fragments (> 50 kb) with one barcode per fragment to create linked reads (creates long distance genome information from short Illumina reads) b) label single cells from a population of cells individually to facilitate high throughput single cell sequencing many applications (e.g. whole genome (de novo) sequencing, haplotype determination, improving of genome drafts, SNP phasing, …)

Instruments for detection, quantification and size selection of nucleic acids

Qubit 3 (Thermo Fisher Scientific)	fluorometer for DNA/RNA quantification sensitivity: down to 100 pg/µl
Quantus Fluorometer (Promega)	dual-channel fluorometer for DNA/RNA quantification sensitivity: down to 10 pg/µl
2100 Bioanalyzer (Agilent)	automated electrophoresis system for DNA/RNA sample quality control and quantification analysis of 1-12 samples per run
TapeStation 4150 (Agilent)	automated electrophoresis system for DNA/RNA sample quality control and quantification analysis of 1-96 samples per run
TapeStation 2200 (Agilent)	automated electrophoresis system for DNA/RNA sample quality control and quantification analysis of 1-96 samples per run
LabChip GX (Caliper Life Sciences)	automated electrophoresis system for DNA/RNA sample quality control and quantification analysis in 96 or 384 plate scale allows PCR based genotyping
Femto Pulse System (Agilent)	automated pulsed field electrophoresis system for DNA/RNA sample quality control and quantification extremely low detection limit – can detect DNA and RNA in fg amounts scalable analysis with broad range of RNA and DNA detection methods
LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer)	automated capillary electrophoresis separation system for DNA/RNA sample quality control and quantification low detection limit (pg amounts) broad variety of analysis options in 96- or 384-well format
CHEF-DR III (Bio-Rad)	pulsed field electrophoresis system high resolution separation of high molecular weight DNA
BluePippin (Sage Science)	DNA size selection with pulsed field electrophoresis (100 bp – 50 kb) handling of 1-5 samples in parallel
SageELF (Sage Science)	DNA sample fractionation with pulsed-field electrophoresis separation into into 12 size fractions allows the construction of libraries with multiple insert sizes for improved assembly

Instruments for lab automation

Biomek i7 (Beckman Coulter)	flexible liquid handling system (8, 96 or 384 scale) 2 pipetting heads, 8 channels thermocycler, shaker, cooling and heating elements integrated working volume: 2-1000 µl
Sciclone G3 (Caliper LifeSciences)	flexible liquid handling system thermocycler, shaker, cooling and heating elements integrated parallel handling of 1-96 samples working volume: 0.5-200 µl
Microlab STARlet (Hamilton)	flexible liquid handling system parallel handling of 1-8 samples working volume: 0.5-1000 µl integration of various devices possible
Biomek FX (Beckman Coulter)	flexible liquid handling system parallel handling of 1-96 samples working volume: 0.5-200 µl integration of various devices possible
Biomek NXp (Beckman Coulter)	flexible liquid handling system parallel handling of up to 384 samples working volume: 0.25-30 µl integration of various devices possible
F5 Robot system (ThermoFisher Scientific)	robot for lab automation transport of plates and consumables 6-axis movement connecting several instruments (thermocycler, centrifuges, carousel, fridge,…)
KingFisher Flex (ThermoFisher Scientific)	instrument for high-throughput extraction and purification of DNA, RNA, protein and cells parallel handling of 24-96 samples

Various other instruments

QIAcuity One (QIAGEN)	one-plate digital PCR (dPCR) instrument detection of up to 5 fluorescent dyes applications: mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, …
ViiA7 (ABI)	real-time PCR system applications: qPCR, Standard curve, Comparative Ct, Genotyping, Presence/Absence
S2 (Covaris)	focused ultrasonicator fragmentation of nucleic acids
Ultimaker3	3D printer fused deposition modeling object size up to 20×20×20 cm supported materials: Nylon, PLA, ABS, CPE, PVA